Posted by James White
Cialis is a very effective drug in treating erectile dysfunction. This medication has many advantages over Viagra and other men’s health drugs. Cialis doesn’t automatically give you an erection. You must be physically or mentally stimulated in order to get this reaction. So, you shouldn’t worry about embarrassing moments with your erection. With Cialis this process is controlled.
Cialis Professional is known for its long lasting effect. It may act in your body for around 36 hours. That is why Cialis is often called ‘the weekend pill’. If you wish to have sex more often, you may take Cialis 5mg every day. It is recommended to take one pill daily around the same time. It’s really easy to swallow a tablet and go about your day.
Unlike other men’s health medications, Cialis Professional is not affected by food or alcohol. Surely, you shouldn’t drink too much alcohol and avoid too fatty foods before the sexual activity.
Cialis would be especially helpful for those guys who are suffering from hypertension, so as it can slightly decrease high blood pressure. Besides, Cialis can also act as an antidepressant.
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Posted by James
There are many men who are not able to perform sexually. One of the reasons why men cannot have a normal sexual life is stress which is the main contributor of impotence. Our life is full of anxieties and worries which often lead to ED and impotence. Nowadays, many men all over the globe face this issue! Due to inadequate blood supply in genital area most men cannot gain a normal erection. Most of them are mistaken, thinking that a problem is only a temporary one. In fact, impotence is a permanent problem if it is not treated. Yet, there is a solution of this problem and it’s called Canadian Pharmacy Levitra. This drug can give you a strong and steady erection and fulfill all your sexual dreams and desires. With this effective and fast medication you don’t need to look for those types of treatment that will go for hours. Levitra is the one that really works for you giving you a chance to achieve a real success!
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Posted by James White
Heat shock also compromised embryonic viability as determined by the total number of cells per embryo at 24 h after the initiation of heat shock. The deleterious effect of heat shock on embryo cell number depended upon the magnitude of heat shock and stage of embryonic development. Thus, heat shock at the 2- to 4-cell stage caused a larger reduction in embryo cell number than heat shock at the morula stage. This finding is in agreement with earlier studies in which heat shock caused a greater reduction in development when applied at the 2-cell stage than the morula stage or at Day 3 after fertilization than at Day 4. Therefore, those embryos that were at stages of development that were capable of heat-induced apoptosis were more resistant to the deleterious effect of heat shock on development.
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Posted by James White
Previous exposure of Day 4 embryos at the 8- to 16-cell stage to a mild and transient heat shock of 40°C for 80 min blocked the induction of apoptosis induced by a subsequent severe heat shock of 41 °C for 9 h. Such a phenomenon has been observed for others cells also. The biochemical mechanisms by which mild heat shock prevents apo-ptosis induced by a more severe heat shock presumably involves HSP70. Besides protecting cells from heat shock, HSP70 can protect cells against several apoptotic stimuli, including DNA damage, UV irradiation, serum withdrawal, and chemotherapeutic agents.
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Posted by James White
Acquisition of heat-induced apoptosis is developmentally regulated and does not occur until approximately Day 4 after insemination at the 8- to 16-cell stage. Spontaneous apoptosis in bovine embryos is also first observed at the 8-to 16-cell stage. The failure to observe apoptosis in 8-to 16-cell embryos collected at Day 3 after insemination may mean that either 1) development of apoptosis mechanisms are controlled by time as well as by the number of cleavage divisions, 2) that Day 4 embryos are more likely to have completed more cleavage divisions than Day 3 embryos, or 3) that Day 4 embryos include retarded embryos that are more susceptible to apoptosis.
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Posted by James White
Heat-Induced Apoptosis in Embryos >16 Cells
Figure 1, A-C, displays representative confocal digital images of embryos that were collected on Day 5 after insemination (if cell number was >16 cells), cultured at either 38.5°C for 24 h or at 41°C for 9 h and 38.5°C for 15 h, and then subjected to the TUNEL reaction. An embryo exposed to 38.5°C is shown in Figure 1A, whereas embryos exposed to 41 °C are shown in Figure 1, B and C. An increase in the proportion of nuclei labeling positive for TU-NEL (yellow in color) is apparent in the embryos exposed to 41°C. The percentage of cells undergoing apoptosis following heat shock was determined in several experiments. In the first experiment, exposure of embryos >16 cells to 41 or 42°C for 9 h increased (P < 0.05) the percentage of cells undergoing apoptosis 24 h after initiation of heat shock compared with embryos cultured at 38.5°C for 24 h (Fig. 2A). Heat shock also tended to reduce (P = 0.07) total number of cells per embryo 24 h after initiation of treatment (Fig. 2B). The effect of heat shock was similar for 41 °C or 42°C.
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Posted by James White
Heat-induced apoptosis in bovine embryos at the 2- or 4-cell stage. Embryos at the 2- or 4-cell stage were collected at 28-30 and 37-39 h after insemination, respectively. Embryos were transferred to a new drop of modified KSOM and cultured at 38.5°C for 24 h or were heat shocked at 41°C for 9 h followed by 38.5°C for 15 h. Embryos were fixed in 4% paraformaldehyde, transferred to a poly-L-lysine coated slide and saved at 4°C until analysis by TUNEL. The experiment was replicated seven times using 491 embryos (94-146 embryos/group).
Heat-induced apoptosis in bovine embryos at the 8- to 16-cell stage on Day 3 after insemination. Embryos at the 8- to 16-cell stage were collected on Day 3 after insemination and transferred to a new drop of modified KSOM. Embryos were then cultured at 38.5°C for 24 h or were heat shocked at 40 or 41°C for 9 h followed by 38.5°C for 15 h. At the end of culture, embryos were fixed and subjected to TUNEL analysis. The experiment was replicated three times using 188 embryos (52-64 embryos/group).
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