Posted by James
Validation of Gentamicin Assay
Three solutions of gentamicin standard (40 mg dissolved and made up to 100 mL in water) were prepared. These solutions were further diluted with water to prepare 3 sets of gentamicin solutions with concentrations of 80, 160, 240, 320, and 400 ^g/mL. Phenylisocyanate derivatives were prepared from samples of each of these solutions, and the resulting solutions were analyzed as described above. These data were used to calculate the linearity and range of the method, with linear regression of response or peak area as a function of concentration.
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Posted by James
Chemicals, Reagents, and Materials
The study was performed between November 2007 and May 2008. Commercially prepared gentamicin 40 mg/mL for injection (Sandoz Canada Inc, Boucherville, Quebec; lot 139819, expiry May 2009), sodium citrate 46.7% for injection (TriCitrasol, Cytasol Laboratories Inc, Braintree, Massachusetts; lot B82T, expiry January 2011), and USP-grade sterile water for injection (Hospira Health Care Corp, Montreal, Quebec; lot 74-337-DK, expiry January 2011) were used to prepare the study solutions, which were stored in polyethylene syringes with luer-lock caps (MedXL Inc, Montreal, Quebec; lot F86331). Gentamicin sulphate USP (PCCA Canada, London, Ontario; lot C104339) and sodium citrate USP (Spectrum, Gardenia, California; lot KC066) were used as reference standards. High-performance liquid chromatography (HPLC)-grade acetonitrile (lot 891499) and phosphoric acid (lot 082037) and analytical-grade hydrochloric acid (lot 4100030) and sodium hydroxide (lot 016045) were purchased from Fisher Scientific (Nepean, Ontario). Hydrogen peroxide USP (6%) was purchased from Pure Standard Products (Edmonton, Alberta; lot 189). Phenylisocyanate (Fluka; lot 1378398), triethylamine (lot 047K1041), and trifluoroacetic acid (Fluka; lot 046710) were purchased from Sigma-Aldrich (St Louis, Missouri). Samples for analysis were stored frozen in cryovials (Nunc, Rochester, New York; lot 089675), and the mobile phase was filtered through Supor-200 polyethersulfone 0.2-^m membranes (Pall, Gelman Laboratories, East Hills, New York; lot 51321).
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Posted by James
INTRODUCTION
Catheter-related infections are a major problem for hemodialysis patients with central venous catheters for vascular access. Bacteremia may result from microorganisms entering the bloodstream at the catheter insertion site or from contamination of the catheter lumen. Various strategies may be used to prevent this complication, including aseptic precautions when manipulating or accessing the catheter and scrupulous hygiene of the skin around the catheter exit site.
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